Axis 4 | Gonad differentiation and physiology

The objective of our research is to better characterize the differentiation of gonadal cells and to decipher the complexity of the expression program, from fetal life to adulthood.

Research in reprotoxicology of our team in humans and rodents has demonstrated the inter-species specificity of biological processes involved in development and gonadal function. In order to better understand the deleterious effects of human viral and chemical exposure on human reproductive health, our research aims at modelling the program of differentiation of human gonadal cells from fetal life to adulthood by characterizing their expression program. In addition to the transcriptomic approaches conventionally used in our laboratory (Affymetrix GeneChips, RNA-seq, DGE-seq), we have acquired in collaboration with the team of Prof. Serge Nef (University of Geneva, Switzerland) a new transcriptome analysis system at the single cell level (single-cell RNA-seq, ChromiumTM system from 10x Genomics). We are currently using this innovative technology to decode the human fetal gonadal expression program to an unprecedented resolution.

Contact: Frédéric Chalmel (CR1 Inserm), Séverine Mazaud-Guittot (CR1 Inserm), Bernard Jégou (DRE Inserm).