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  1. Chercheur post-doctoral en chimie analytique
  2. PhD position | Metabolomics to characterize the human chemical exposome
  3. PhD position | Studies of Endocrine disrupter pollutants in zebrafish embryos: Contribution of genetically modified models (transgenic, Knock out) to predict toxicological impacts
  4. PhD position | Role of EXOSC10 during folliculogenesis and oogenesis
  5. PhD position | Role of TRIM21 in TRAIL-induced necroptosis and in hepatocarcinoma progression
  6. PhD position | Antiviral immunity in the testis

Chercheur post-doctoral en chimie analytique

Date de publication : 24 mai 2018

Poste au Laboratoire d’étude et de recherche en environnement et santé (Leres, EHESP)

Contrat : CDD de 18 mois, à partir de septembre 2018

Date de clôture des dépôts de candidatures : 15 juin 2018
 

 

PhD position | Metabolomics to characterize the human chemical exposome

Publication date: 3 May 2018

Description
The French School of Public Health, a member of the University Sorbonne-Paris Cité (http://www.sorbonne-paris-cite.fr/), seeks highly qualified candidates for a PhD position to work in the field of metabolomics/exposomics. The Exposome represents all environmental exposures from conception onwards and includes broad categories of exposure, e.g. pollutant exposure, gut microflora, diet, lifestyle factors, and encompasses the wider social, economic and psychological influences. This new concept has recently emerged to study the increasing prevalence of human non communicable diseases (e.g, cancer, metabolic disorders, obesity, reproductive disorders), a major cause of mortality worldwide. [...]

Scientific and technical skills required by the candidate
We seek a highly motivated and enthusiastic candidate with a master in the area of metabolomics, environmental chemistry, or analytical chemistry with experience and demonstrated success of working independently and as part of a team in analytical or academic research facility. The successful applicant will work at the Laboratory for Research and Study in Environment and Health (Leres), a major analytical platform fully integrated within Irset-Inserm UMR 1085. Essential skills for this job include experience in biological sample preparation techniques, strong practical expertise in liquid chromatographic methods, LC-HRMS based metabolomics and experience in metabolite deconvolution software and Mass Spectral Database.
The successful applicant will also have to develop and implement a data processing pipeline including experimental design, pre-processing (bioinformatics), and statistical analysis of large scale LC-MS based metabolomics studies. Experience with computer programming (e.g. R, Matlab) and multivariate statistics (e.g. SIMCA) would be highly advantageous for this job. The position will include collaborations with epidemiologists, toxicologists and developmental biologist within the Research Institute for Environmental and Occupational Health (Irset-Inserm UMR 1085) and Rigshospitalet (Copenhagen, Denmark). Effective communication skills, both oral and written is required.

Application
The successful candidate is expected to start in September 2018. The closing date for applications is 31 May 2018. Applicants are invited to email their application including CV, an application letter, diploma and a list of 2-3 referees to Dr Arthur David (arthur.david@ehesp.fr). Interested applicants can email Dr Arthur David for further information.

 

PhD position | Studies of Endocrine disrupter pollutants in zebrafish embryos: Contribution of genetically modified models (transgenic, Knock out) to predict toxicological impacts

Publication date: 27 April 2018

Description
Endocrine disruptors are molecules that can affect the expression of hormone-regulated genes. Early exposures during developmental stages are likely to lead to long term effects. We previously highlighted environmental pollutant-linked transcriptional dysregulation of genes involved in the hormonal biosynthesis in the central nervous system during the embryonic development of zebrafish (Brion et al., 2012; Vosges et al., 2012; Vosges et al., 2010; Cano-Nicolau et al., 2016). The question of the long term consequences of these pollutants on central estrogenic signaling remains to be explored. This thesis aims at understanding how the developmental regulation of estrogen receptors and cerebral aromatase expression affect the long term organization of the central nervous system, using zebra fish models. Such an approach is made possible thanks to the recent development of knockout lines for the ERs receptors and Cyp19a1b available in our laboratory. The prediction of developmental effects also implies integrative approaches combining spatio-temporal analysis of the expression of target genes and physiological process modulated by the presence of neuro-steroids. The candidate will also work toward the integration of these biological and toxicological data into mathematical models (quantitative Adverse Outcome Pathway, qAOP) to predict the (eco) -toxicological impacts of endocrine disruptors on human and environmental health (collaboration with F. Bois, Modeling Unit in France, toxicology and ecotoxicology, as part of the EU-ToxRisk project). The thesis will be co-supervised by Francois Brion (Ineris) and Thierry Charlier (University of Rennes 1, Irset).

Location
Ineris (National Institute of Industrial Environment and Risks is a Public Institution of Industrial and Commercial nature (EPIC) placed under the supervision of the Ministry of Ecological and Solidarity Transition in France. Its mission is to carry out or commission studies and research to evaluate and prevent accidental or chronic risks to humans and to the environment, and more particularly those risk related to industrial installations, chemical substances and underground operations. The head office of Ineris is located in Verneuil-en-Halatte, on a park of more than 40 hectares in a privileged green setting, 40 minutes from Paris and 10 minutes from Senlis / Chantilly (A1) - 30 minutes from Paris Nord + shuttle bus.
This project will be developed in collaboration with Irset. Irset is a joint research unit of the Inserm institute, Rennes 1 University and the EHESP - School of Public Health, in partnership with the Universities of Angers and the French Antilles, the CNRS, and the Teaching Hospitals of Rennes, Angers and Pointe-à-Pitre. Its mission is to study the biological processes and the environmental factors (whether chemical, biological, physical, social and cultural, occupational, geographical or economic) that affect human health, and to help public health authorities make informed decisions on the basis of scientific data.

Scientific and technical skills required by the candidate
The potential candidate should have a master or equivalent in biology. Previous experience in laboratory setting is mandatory (Practical knowledge in cell biology, biochemistry and/or physiology).

Application
Application, including transcripts, CV and motivation letter should be sent to Francois Brion (Francois.BRION@ineris.fr) and Thierry Charlier (thierry.charlier@univ-rennes1.fr) or can be uploaded on the Ineris web site.

PhD position | Role of EXOSC10 during folliculogenesis and oogenesis

Publication date: 18 April 2018

This PhD position is available for a period of 3 years at Irset/InsermU1085, University of Rennes 1 (Rennes, Brittany, France)

Funding: University of Rennes 1

Starting date: October 2018

Project:
About 15-20% of couples are affected by a fertility problem and in 40% of cases the origin is exclusively related to women. The molecular causes are often unknown and a possible implication of a new class of RNAs – long non-coding RNAs (lncRNA) – is not understood. EXOSC10 is one of two conserved subunits of the nuclear exosome involved in the degradation and alteration of all RNAs. It has been shown that, in humans, EXOSC10 is essential for mitosis in cultured cells. We have previously identified the essential role of this protein during spermatogenesis in mice. In addition, our current studies indicate that EXOSC10 is necessary for early embryonic development of the mouse. The aim of this project is to examine the role of EXOSC10 during mouse oogenesis and to determine if this protein is involved in key stages of this process. We propose an in vivo study by inactivating Exosc10 gene specifically in the oocyte and in granulosa cells. After a phenotypic analysis, we plan to perform a high throughput analysis (RNA-seq) to identify the Exosc10-dependent pathways during oogenesis and folliculogenesis. Potential candidates will be further studied using in situ hybridization, q-PCR and primary cell culture.

Profile:

  • Master 2 degree in Cellular and Molecular Biology or Reproduction and Development or Biology of Reproduction, obtained in 2018. For your application to be considered, you need to be ranked among the top third of your Master 2.
  • Skills and knowledge in molecular biology (PCR, qPCR), cellular biology (immunohistochemistry, in situ hybridization, western blot) and cell culture are necessary. The applicant will have to take a course on animal research.

We seek a highly motivated and talented PhD student. The candidate should be capable of developing a competitive research project while working in autonomy under the supervision of a senior researcher.
More information: https://www.irset.org/en/axis-2-regulation-cell-division-during-developm...

How to apply:
Send your CV with a motivation letter and the contact details of two referees electronically to Drs Soazik Jamin (soazik.jamin@inserm.fr) and Fabrice Petit (fabrice.petit@inserm.fr).
Application deadline for pre-selection is 4 June 2018.
The selected candidates will hold a competitive oral presentation in front of the Biology-Health Doctoral school jury on 27, 28 or 29 June 2018 (video conference possible).

 

PhD position | Role of TRIM21 in TRAIL-induced necroptosis and in hepatocarcinoma progression

Publication date: 12 April 2018

This PhD position is available for a period of 3 years at Irset, University of Rennes 1 (Brittany, France)

Funding: University of Rennes 1

Starting date: October 2018

Project:
In 90% of cases, hepatocellular carcinoma (HCC), regardless of their etiology (virus, alcohol, obesity), occur on fibrous or cirrhotic livers. In this context, the death of hepatocytes could be the trigger for the disease progression. Our team has recently implemented an in vivo model of diabetic mice submitted to high fat diet leading to the development of HCC. In parallel, we have already developed research on TRAIL (TNF-Related Apoptosis-Inducing Ligand), a cytokine of the TNF family (Tumor Necrosis Factor) involved in innate immunity. TRAIL induces cell death by apoptosis but also by necroptosis in the presence of zVAD (a caspase inhibitor) and Birinapant (Bp, an inhibitor of IAPs, Inhibitors of Apoptosis). Necroptosis is considered as an inflammation-inducing cell death by the release of DAMPs (Damage-Associated Molecular Patterns).The aim of this thesis project is to better understand the role of TRIM21 (tripartite motif-containing protein 21) in TRAIL / zVAD / Bp-induced necroptosis signaling and the molecular processes that control the transition from inflammation to liver cancer. The successful candidate will perform original research using cellular and molecular biology, unique NASH / diabetes mouse model of HCC as well as TRIM21-deficient cells and TRIM21-deficient mice.  

Profile:

  • Master 2 degree in Cancerology, Immunology or Cellular and Molecular Biology, obtained in 2018. For your application to be considered, you need to be ranked among the top third of your Master 2.
  • Skills and knowledge in cell culture and molecular biology (immunoprecipitation, cloning, cellular transfection) are necessary. The candidate should master at least part of the techniques mentioned above. Previous experience in an animal lab will be a bonus.

We are looking for a highly motivated and talented PhD student. The candidate should be creative and capable of developing a competitive research project while working in autonomy under the supervision of senior scientists and with a team spirit.

How to apply:
Send your CV with a motivation letter and the contact details of two referees electronically as one PDF-file to Drs Marie-Thérèse Dimanche-Boitrel (marie-therese.boitrel@univ-rennes1.fr) and Jacques Le Seyec (jacques.le-seyec@univ-rennes1.fr).
Application deadline for pre-selection is 13 May 2018.
The selected candidates will hold a competitive oral presentation in front of the Biology-Health Doctoral school jury on 28-29 June 2018 (videoconference possible).

 

PhD position | Antiviral immunity in the testis

Publication date: 10 April 2018

This PhD position is available for a period of 3 years at Irset, University of Rennes 1 (Brittany, France)

Funding: University of Rennes 1

Starting date: October 2018

Project:
Viral infection of the testis can have a number of deleterious consequences including infertility caused by the inflammation of the organ in response to the infection (e.g. mumps virus infection). Infection of the male genital tract also represents a population-wide threat, semen being an important vector for the spread of viruses. Of concern, the sexual transmission and persistence in semen of emerging viruses (e.g. Zika virus) was recently reported. Our recent data indicate a tropism of Zika virus for the human testis. The aim of this thesis project is to better understand the molecular mechanisms of viral persistence and inflammation in the human testis. The successful candidate will perform original research using unique organotypic culture of human tissue developed in the laboratory, which will be infected ex vivo. The organ innate immune response will be deciphered using single cell transcriptomic analysis, in situ hybridization, luminex, flow cytometry and siRNA/CRISPR/CAS9 extinction in primary cell culture.

Profile:

  • Master 2 degree in Virology, Immunology or Cellular and Molecular Biology, obtained in 2018. For your application to be considered, you need to be ranked among the top third of your Master 2.
  • Skills and knowledge in cell culture, in primary cell/organ culture, molecular biology and virology are necessary. The candidate should master at least part of the techniques mentioned above. Previous experience in a security lab L2/L3 will be a bonus.

We seek a highly motivated and talented PhD student. The candidate should be creative and capable of developing a competitive research project while working in autonomy under the supervision of a team leader.

How to apply:
Send your CV with a motivation letter and the contact details of two referees electronically as one PDF-file to Drs Nathalie Dejucq-Rainsford (nathalie.dejucq-rainsford@inserm.fr) and Anna Le Tortorec (anna.letortorec@univ-rennes1.fr).
Application deadline for pre-selection is 13 May 2018.
The selected candidates will hold a competitive oral presentation in front of the Biology-Health Doctoral school jury on 28-29 June 2018 (videoconference possible).