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  1. PhD position: Antiviral immunity in the testis
  2. PhD position: Role of TRIM21 in TRAIL-induced necroptosis and in hepatocarcinoma progression

PhD position: Antiviral immunity in the testis

Publication date: 10 April 2018

This PhD position is available for a period of 3 years at Irset, University of Rennes 1 (Brittany, France)

Funding: University of Rennes 1

Starting date: October 2018

Project:
Viral infection of the testis can have a number of deleterious consequences including infertility caused by the inflammation of the organ in response to the infection (e.g. mumps virus infection). Infection of the male genital tract also represents a population-wide threat, semen being an important vector for the spread of viruses. Of concern, the sexual transmission and persistence in semen of emerging viruses (e.g. Zika virus) was recently reported. Our recent data indicate a tropism of Zika virus for the human testis. The aim of this thesis project is to better understand the molecular mechanisms of viral persistence and inflammation in the human testis. The successful candidate will perform original research using unique organotypic culture of human tissue developed in the laboratory, which will be infected ex vivo. The organ innate immune response will be deciphered using single cell transcriptomic analysis, in situ hybridization, luminex, flow cytometry and siRNA/CRISPR/CAS9 extinction in primary cell culture.

Profile:

  • Master 2 degree in Virology, Immunology or Cellular and Molecular Biology, obtained in 2018. For your application to be considered, you need to be ranked among the top third of your Master 2.
  • Skills and knowledge in cell culture, in primary cell/organ culture, molecular biology and virology are necessary. The candidate should master at least part of the techniques mentioned above. Previous experience in a security lab L2/L3 will be a bonus.

We seek a highly motivated and talented PhD student. The candidate should be creative and capable of developing a competitive research project while working in autonomy under the supervision of a team leader.

How to apply:
Send your CV with a motivation letter and the contact details of two referees electronically as one PDF-file to Drs Nathalie Dejucq-Rainsford (nathalie.dejucq-rainsford@inserm.fr) and Anna Le Tortorec (anna.letortorec@univ-rennes1.fr).
Application deadline for pre-selection is 13 May 2018.
The selected candidates will hold a competitive oral presentation in front of the Biology-Health Doctoral school jury on 28-29 June 2018 (videoconference possible).

 

PhD position: Role of TRIM21 in TRAIL-induced necroptosis and in hepatocarcinoma progression

Publication date: 12 April 2018

This PhD position is available for a period of 3 years at Irset, University of Rennes 1 (Brittany, France)

Funding: University of Rennes 1

Starting date: October 2018

Project:
In 90% of cases, hepatocellular carcinoma (HCC), regardless of their etiology (virus, alcohol, obesity), occur on fibrous or cirrhotic livers. In this context, the death of hepatocytes could be the trigger for the disease progression. Our team has recently implemented an in vivo model of diabetic mice submitted to high fat diet leading to the development of HCC. In parallel, we have already developed research on TRAIL (TNF-Related Apoptosis-Inducing Ligand), a cytokine of the TNF family (Tumor Necrosis Factor) involved in innate immunity. TRAIL induces cell death by apoptosis but also by necroptosis in the presence of zVAD (a caspase inhibitor) and Birinapant (Bp, an inhibitor of IAPs, Inhibitors of Apoptosis). Necroptosis is considered as an inflammation-inducing cell death by the release of DAMPs (Damage-Associated Molecular Patterns).The aim of this thesis project is to better understand the role of TRIM21 (tripartite motif-containing protein 21) in TRAIL / zVAD / Bp-induced necroptosis signaling and the molecular processes that control the transition from inflammation to liver cancer. The successful candidate will perform original research using cellular and molecular biology, unique NASH / diabetes mouse model of HCC as well as TRIM21-deficient cells and TRIM21-deficient mice.  

Profile:

  • Master 2 degree in Cancerology, Immunology or Cellular and Molecular Biology, obtained in 2018. For your application to be considered, you need to be ranked among the top third of your Master 2.
  • Skills and knowledge in cell culture and molecular biology (immunoprecipitation, cloning, cellular transfection) are necessary. The candidate should master at least part of the techniques mentioned above. Previous experience in an animal lab will be a bonus.

We are looking for a highly motivated and talented PhD student. The candidate should be creative and capable of developing a competitive research project while working in autonomy under the supervision of senior scientists and with a team spirit.

How to apply:
Send your CV with a motivation letter and the contact details of two referees electronically as one PDF-file to Drs Marie-Thérèse Dimanche-Boitrel (marie-therese.boitrel@univ-rennes1.fr) and Jacques Le Seyec (jacques.le-seyec@univ-rennes1.fr).
Application deadline for pre-selection is 13 May 2018.
The selected candidates will hold a competitive oral presentation in front of the Biology-Health Doctoral school jury on 28-29 June 2018 (videoconference possible).